PrecisionBind Human Interleukin 21 (IL-21 / IL21) ELISA (NIBSC/WHO calibrated)

PrecisionBind Human Interleukin 21 (IL-21 / IL21) ELISA (NIBSC/WHO calibrated)

SKU: KB1085

Rs 38,000.00

Enzyme Immunoassay for the estimation of Interleukin 21 (IL-21 / IL21) in human serum, plasma, cerebrospinal fluid and other biological samples.

PrecisionBind ELISA are matrix-evaluated cytokine immunoassays designed for immune monitoring and translational research, with reference-standard calibration (NIBSC/WHO where available) and performance documentation aligned to applied bioanalysis (ICH M12 for linearity, recovery, precision, and matrix effects). RUO only. Sample matrices Validated: Serum, Plasma, Cerebrospinal Fluid, Cell Culture Supernatant and Cell / Tissue Lysates

Assay Range: 15.6 – 1000 pg/ml | Sensitivity: Analytical Sensitivity: LOD 2 pg/ml | Functional Sensitivity: LOQ5 pg/ml | IC50%~440 pg/ml

Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells, break-apart

 

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All our kits are manufactured under ISO 13485.

The precisionbind Human Interleukin 21 (IL-21 / IL21) ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Principle Of Assay: This ELISA is a sandwich immunoassay. Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples.

Disclaimer: The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit.

Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. The The precisionbind ELISA kits are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies – capture and detection to ensure a high degree of sensitivity and specificity in the estimation of Interleukin 21 (IL-21 / IL21);

About PrecisionBind:
PrecisionBind is Krishgen`s cytokine ELISA platform developed for bioanalytical robustness in translational research, including immuno-oncology and cell & gene therapy programs. Where applicable, kit standards are calibrated against NIBSC/WHO international standards to support unit traceability and inter-study comparability.

Assays are characterized following ligand-binding assay performance expectations, including:

  • Defined assay range and sensitivity (LOD/LOQ where reported)
  • Intra- and inter-assay precision
  • Cross-matrix performance characterization (serum, plasma, CSF where applicable), including parallelism and recovery assessment, to support quantitative comparability across biological matrices.

    All kits are Research Use Only (RUO) and not intended for diagnostic use.

    • Additional information

    Pack Type

    1 x 96 wells, break-apart
    Species Reactivity

    Human
    Sample Type

    Serum, Plasma, Cell Lysates, Cerebrospinal Fluid, Cell Culture Supernates and other biological fluids. K2EDTA serum and plasma validated only. For tear samples, and other plasmas, please connect with us to discuss further.
    Assay Range

    15.6 – 1000 pg/ml
    Standards Available

    0; 15.625; 31.25; 62.5; 125; 250; 500; 1000 pg/ml
    Sensitivity

    Analytical Sensitivity: LOD 2 pg/ml | Functional Sensitivity: LOQ5 pg/ml | IC50%~440 pg/ml
    Principle Of Assay

    Sandwich ELISA, Double Antibody
    Detection Method

    Absorbance measured at 450nm, Colorimetric
    Conjugate-Enzyme Reaction

    and dynamic range, balancing speed, sensitivity
    Regulatory Status

    For Research Use Only (RUO).
    Shipping Temperature

    Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius. Some components may be shipped to -20 degrees Celsius
    Storage Temperature

    if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results
    ELISA Interpretation

    4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, Quantitative Results Intepretation, to support interpretation of results.
    Shelf Life Available

    8 -10 months at time of shipping
    Alternate Names / Synonyms

    Interleukin 21; IL-21; IL21; CVID11, Interleukin-21, Za11
    Research Area

    Immune Molecule
    Disclaimer

    The data indicated herein with specifications are changed from time to time at time of production. Please refer the most current IFU (instructions for use) accompanying the kit.
    SKU: KB1085 Category:

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