Introduction:
The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.
Intended Use:
The GENLISA Equine Pregnant Mare Serum Gonadotrophin, PMSG ELISA is used as an analytical tool for quantitative determination of Equine Pregnant Mare Serum Gonadotrophin, PMSG in serum, plasma and other biological samples.
Principle:
The method employs sandwich ELISA technique. Equine Pregnant Mare Serum Gonadotrophin, PMSG monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Equine Pregnant Mare Serum Gonadotrophin, PMSG present in the sample are bound by the antibodies. Biotin labeled PMSG antibody is added and followed by Streptavidin-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Equine Pregnant Mare Serum Gonadotrophin, PMSG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
