GENLISA Human Alpha-1-B-Glycoprotein (A1BG) ELISA

SKU: KBH1061

Rs 30,000.00

Enzyme Immunoassay for the estimation of Human Calcitonin Gene Related Peptide (CGRP) ELISA in serum, plasma, cell culture supernatant and other biological samples.


Assay Range: 0.312 – 20 ng/ml | Sensitivity: 0.147 ng/ml

Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells, break-apart

 

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All our kits are manufactured under ISO 13485.

The GENLISA Human Pulmonary Surfatcant-Associated Protein B (SP-B) ELISA includes features like: • Ready to use protocol with break-apart wells for ease of use • Standardisation and High Reproducibility • Lot to Lot Consistency • Accuracy and Precision
Principle Of Assay: This ELISA is a sandwich immunoassay. Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples.

Disclaimer: The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit. Validated against seven points for a “GOLD RING” Standard Quality ELISA the benchmark sign for Krishgen Quality. The GENLISA ELISAs are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant and uses monoclonal antibodies to ensure a high degree of specificity and reagents of superior quality to offer a sensitive assay. For project based larger number ofs, talk to us to offer you our validation guide for the same

Additional information

Pack Type

1 x 96 wells, break-apart

Species Reactivity

Human

Sample Type

Serum, Plasma, Cell Culture Supernatant and other biological samples. Validated for citrated/EDTA Plasma only. Check with us if you wish validation data for CSF or tear fluids or any other specific matrices.

Assay Range

0.312 – 20 ng/ml

Standards Available

0; 0.312; 0.625; 1.25; 2.5; 5; 10; 20 ng/ml

Sensitivity

0.147 ng/ml

Principle Of Assay

Sandwich ELISA, Double Antibody

Detection Method

Absorbance measured at 450nm, Colorimetric

Conjugate-Enzyme Reaction

and dynamic range, balancing speed, sensitivity

Regulatory Status

For Research Use Only (RUO).

Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius. Some components may be shipped to -20 degrees Celsius

Storage Temperature

if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results

ELISA Interpretation

4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, Quantitative Results Intepretation, to support interpretation of results.

Shelf Life Available

8 -10 months at time of shipping

Alternate Names / Synonyms

Alpha-1-B-Glycoprotein (A1BG); A1BG; Alpha-1B-glycoprotein; Alpha-1-B glycoprotei

Research Area

Immune Molecule

Disclaimer

The data indicated herein with specifications are changed from time to time at time of production. Please refer the most current IFU (instructions for use) accompanying the kit.