GENLISA Human Non Ester Fatty Acid (NEFA) / Free Fatty Acid (FFA) ELISA

GENLISA Human Non Ester Fatty Acid (NEFA) / Free Fatty Acid (FFA) ELISA

SKU: KBH3347

Rs 30,000.00

Enzyme Immunoassay for the estimation of Human Non Ester Fatty Acid (NEFA) / Free Fatty Acid (FFA) in serum, plasma, tissue homogenates, saliva and other biological fluids as validated.

Assay Range: 6.25 – 400 nmol/ml. *Using oleic acid (mw 282.47) as the standard reference ( 1765.44 – 112,988 ng/ml) | Sensitivity: 3 nmol/ml

Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells, break-apart

 

Guaranteed Safe Checkout

Accepted payment methods

All our kits are manufactured under ISO 13485.

Introduction: The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a comptetitive ELISA technique.

Intended Use: The GENLISA Human Non Ester Fatty Acid (NEFA) / Free Fatty Acid (FFA) ELISA kit is used as an analytical tool for quantitative determination of Gastrin in serum, plasma and other biological samples.

Principle: The method employs competitive ELISA technique. Antibody: HRP conjugate is added as the final addition. After washing microwells in order to remove any non-specific binding, the Chromogenic substrate solution is added to microwells and color develops inversely proportionally to the amount of human Non Ester Fatty Acid (NEFA) / Free Fatty Acid (FFA) in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Additional information

Pack Type

1 x 96 wells, break-apart
Species Reactivity

Human
Sample Type

Serum, Plasma, Cell Culture Supernatant and other biological samples. Validated for citrated/EDTA Plasma only. Check with us if you wish validation data for CSF or tear fluids or any other specific matrices.
Assay Range

6.25 – 400 nmol/ml. *Using oleic acid (mw 282.47) as the standard reference ( 1765.44 – 112, 988 ng/ml)
Standards Available

0; 6.25; 12.5; 25; 50; 100; 200; 400 nmol/ml
Sensitivity

3 nmol/ml
Principle Of Assay

Competitive ELISA, Coated with Antibody
Detection Method

Absorbance measured at 450nm, Colorimetric
Conjugate-Enzyme Reaction

HRP (Horseradish Peroxidase) conjugate with TMB substrate
Regulatory Status

For Research Use Only (RUO).
Shipping Temperature

Shipped at ambient temperature or with gel packs to maintain 2-8 ?C. Some components may be shipped at -20 ?C.
Storage Temperature

Store the kit at 2-8 ?C. Return unused microwell strips to the foil pouch with the desiccant pack and reseal. Store individual components as indicated in the IFU (Instructions for Use).
ELISA Interpretation

4-Parameter Logistic (4PL) curve fit or cubic spline. An MS Excel-based template for quantitative interpretation of results is available on request.
Shelf Life Available

8 -10 months at time of shipping
Alternate Names / Synonyms

FFA; free fatty acid; NEFA; non ester fatty free acid
Research Area

Immune Molecule
Disclaimer

The data indicated herein with specifications are changed from time to time at time of production. Please refer the most current IFU (instructions for use) accompanying the kit.
SKU: KBH3347 Category: