Frequently Asked Questions
Which region(s) of human pro-amylin (pro-IAPP) are recognized by the capture and detection antibodies?
N-terminal pro-peptide region of Pro-Amylin.
Are the antibody epitopes located within the N-terminal propeptide, the mature amylin sequence, the C-terminal propeptide, or at a prohormone processing junction?
Only intact full-length Pro-Amylin is detected; partially processed intermediates will not produce valid signals.
Does the assay specifically detect intact pro-amylin, or does it also detect partially processed pro-amylin intermediates?
There is nearly no cross-reactivity with mature amylin.
The GENLISA Human Pro-Amylin ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision Validated against seven points for a “GOLD RING” Standard Quality ELISA
– the benchmark sign for Krishgen Quality. The GENLISA ELISA kits are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies – capture and detection to ensure a high degree of sensitivity and specificity in the estimation of Pro-Amylin;
