| Pack Type |
1 x 96 wells, break-apart wells
|
| Species Reactivity |
Homo Sapiens (Human)
|
| Sample Type |
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. For tear samples, please connect with us to discuss further. Note the kit has been validated using human matrices only. For any other animal matrix, pleas connect with us to optimize the kit accordingly.
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| Biomarker / Protein |
SARS-CoV-2 spike protein S1 Antigen
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| Sample Dilution |
Dilute at 1:1000 with the Sample Diluent provided in the kit. Please refer to the IFU enclosed with the kit supplied for specific guidance.
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| Human SARS-CoV-2 (Covid-19) spike protein S1 Antigen Quantitative ELISA |
Short Name
|
| Standards Available |
0; 62.5; 125; 250; 500; 1000; 2000; 4000 ng/ml
|
| Calibration Curve |
6/8 point; standards available
|
| Sensitivity |
62.5 ng/ml
|
| Interference |
Preparations of the known factors were assayed for interference. No significant interference was observed.
|
| Specificity |
The assay uses anti idiotypic (humanized) antibody for capture and / or recombinant protein expressed in mamalian cells, The standards used are research grade biosimilars referenced to the innovator product. In specfic cases where vailable the kit has also been validated against the innovator / reference product. Refer our detailed validation guide for the same.
|
| Principle Of Assay |
This ELISA is a sandwich immunoassay. Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples.
|
| Detection Method |
Colorimetric, Absorbance measured at 450nm
|
| Conjugate-Enzyme Reaction |
Uses stable HRP Enzyme Conjugate with (single component kinetic) and TMB Chromogenic Substrate for color development.
|
| Regulatory Status |
Research Use Only. Kit is developed and validated as per ICH/EMA/FDA guidelines. Ask for our detailed validation guide at sales1@krishgen.com
|
| Shipping Temperature |
Shipped at Room Temperature or in gel pack
|
| Storage Temperature |
The kit and its components are to be stored at 2-8 Degree Celsius. Please refer specific components storage conditions in the IFU.
|
| ELISA Type |
Sandwich ELISA, Double Antibody
|
| ELISA Interpretation |
Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results.
|
| Product Catalog |
Infectious Disease
|
| Therapeutic / Biology Type |
Infectious Disease
|
| Biological Pathway |
Antiviral immune response
|
| KEGG Target Pathway |
hsa05171
|
| Reactome Pathway ID |
-HSA-9679506
|
| Active Molecule |
SARS-CoV-2 (Covid-19) spike protein S1 Quantitative
|
| Shelf Life Available |
8 -10 months at time of shipping
|
| Alternate Names / Synonyms |
SARS-CoV-2; Covid-19; Spike protein S1 Antigen Quantitative
|
| Research Area |
Infectious Disease, Coronavirus
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| Disclaimer |
The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit. Trade name indicated is for reference purposes only. It does not reflect any licences or patent usage. The tradename is the registered trademark of the respective owners only. References towards clinical trials, pathways, research areas are for informational purpose only.
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