Introduction:
Japanese encephalitis virus (JEV) is an enveloped, single-stranded RNA virus which belongs to the genus Flavivirus within the family Flaviviridae. JEV occurs as a single serotype but has considerable antigenic variation. Scientists have been isolated more than 50 strains in Japan. PCR, RT-LAMP, ELISA, and immunochromatography are the diagnostic methods currently used to detect JEV.
Intended Use:
The GENLISA Japanese Encephalitis (JE) Antigen ELISA is used as an analytical tool for quantitative determination of JEV Envelope Protein in human serum and plasma.
Principle:
The method employs the quantitative sandwich enzyme immunoassay technique. JEV Envelope Antibody is pre-coated onto microwells. Samples and standards are pipetted into microwells and JEV Envelope Protein present in the sample are bound by the capture antibody.Then a HRP (horseradish peroxidase) conjugated Japanese Encephalitis Virus Antibody is pipetted and incubated. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of JEV Envelope Protein in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.
