GENLISA Rat Symmetric Dimethylarginine (SDMA) ELISA

GENLISA Rat Symmetric Dimethylarginine (SDMA) ELISA

SKU: KLR1615

Rs 32,000.00

Enzyme Immunoassay for the estimation of Rat Symmetric Dimethylarginine (SDMA) ELISA in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids as validated.

Assay Range: 0.156 – 10 ng/ml | Sensitivity: 0.055 ng/ml

Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells, break-apart

 

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All our kits are manufactured under ISO 13485.

This GENLISA Rat Symmetric Dimethylarginine (SDMA) ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. This GENLISA ELISA kit is used for assessing the specific biomarker in samples analytes which may be serum, plasma, tissue homogenates and other biological fluids. The kit uses monoclonal antibodies to ensure a high degree of sensitivity and specificity in the estimation of the analyte.

Additional information

Pack Type

1 x 96 wells, break – apart wells
Species Reactivity

Rat
Sample Type

Serum, plasma, tissue homogenates and other biological fluids.
Short Name

Rat Symmetric Dimethylarginine (SDMA) ELISA
Assay Range

0.156 – 10 ng/ml
Sensitivity

0.055 ng/ml
Standards Available

0; 0.15625; 0.3125; 0.625; 1.25; 1.25; 2.5; 5; 10 ng/ml
Calibration Curve

8 point; standards available
Interference

Preparations of the known factors were assayed for interference. No significant interference was observed.
Specificity

This assay recognises both the recombinant and natural protein.
Principle of Assay

This ELISA is a competitive inhibition assay (also called a competitive ELISA) is a powerful assay format used to detect small antigens or haptens that cannot be simultaneously bound by two antibodies (as in sandwich ELISA). In competitive ELISA, the sample / standard antigen competes with a labeled antigen for a limited amount of capture monoclonal antibody. The more antigen present in the sample, the less labeled antigen is bound, resulting in a lower signal. So, signal intensity is inversely proportional to the concentration of the target antigen.
Detection Method

Colorimetric, Absorbance measured at 450nm
Conjugate-Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development.
Regulatory Status

Research Use Only.
Shipping Temperature

Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius
Storage Temperature

The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity.
ELISA Type

Competitive ELISA, Coated with Antigen
ELISA Interpretation

Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results.
Shelf Life Available

8 -10 months at time of shipping
Alternate Names / Synonyms

Rat Symmetric Dimethylarginine; SDMA
Disclaimer

The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit.
SKU: KLR1615 Category: