The kit includes the following components:
– brucellosis antigen for the RBT. It is a suspended matter of inactivated brucellas of species abortus in lactic buffer solution pH 3.65 with 0.5% phenol, Rose Bengal-stained;
– dry brucellosis serum is a blood serum of bulls (oxen) hyperimmunized by a live culture of brucellas of species abortus;
– dry negative blood serum of cattle.
Assay Principle: The S-brucellosis antigen for the milk ring test is agglutinated by homologous antibodies of milk of cows that are diseased with brucellosis or immunized with brucellosis agglutinogenic vaccines resulting in phenomenon of adsorption of the antigen-antibody complex by milk fat globules and formation of the coloured ring in the cream layer. The brucellosis serum contains specific agglutinins. It is used as a positive control for the milk ring test.
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Additional information
| Sample Type | Serum |
|---|---|
| Assay Type | Qualitative, screening assay |
| Sensitivity | ~>99% |
| Interference | Preparations of the known factors were assayed for interference. No significant interference was observed. |
| Specificity | This assay recognises both the recombinant and natural protein |
| Detection Method | Visual |
| Test Reaction | Agglutination |
| Quality Certification | Validated as per KRISHGEN's Quality Program |
| Regulatory Status | For In-Vitro Testing Only |
| Results Interpretation | Qualitative Results intepretation on basis of turbidity. |
| Shelf Life Available | 10-12 months at time of shipping |
| Alternate Names / Synonyms | Brucellosis, RBT, Rose Bengal Test |