KRIBIOLISA Anti Abaloparatide (TYMLOS) ELISA

Name: KRIBIOLISA Anti Abaloparatide (TYMLOS) ELISA
SKU: KBI9055
Availability: InStock

SKU: KBI9055

Rs 83,000.00

Enzyme Immunoassay for the estimation of Anti Abaloparatdie (TYMLOS) in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Range: 0 – 2000 ng/ml | Sensitivity: 15 ng/ml

Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells, break-apart

Description
Additional information

Background: Abaloparatide is a synthetic analog of human parathyroid hormone-related peptide (PTHrP 1–34) developed for the treatment of osteoporosis and other bone metabolism disorders. It acts as an anabolic agent by selectively activating the parathyroid hormone 1 receptor (PTH1R), thereby stimulating osteoblastic bone formation and increasing bone mineral density. Anti Abaloparatide ELISA is designed to measure the antibodies against Abalopratide.

KRIBIOLISA Anti Abaloparatide ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Validated against seven points for a “GOLD RING” Standard Quality ELISA – the benchmark sign for Krishgen Quality. KRIBIOLISA ELISA kit is used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies / recombinant proteins – capture and detection to ensure a high degree of sensitivity and specificity in the estimation of the analyte. Extensive validation has been done on these kits. Please ask for our validation guide if not availableon our product page. Incase you wish us to customize the kit, connect with us at sales1@krishgen.com

Additional information

Pack Type	1 x 96 wells, break-apart
Product Catalog	Metabolic Hormones
Assay Range	0 – 2000 ng/ml
Sensitivity	15 ng/ml
Sample Type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. For tear samples, please connect with us to discuss further. Note the kit has been validated using human matrices only. For any other animal matrix, pleas connect with us to optimize the kit accordingly.
Species Reactivity	Homo Sapiens (Human)
Biomarker / Protein	Abaloparatide
Interference	Preparations of the known factors were assayed for interference. No significant interference was observed.
Principle Of Assay	This ELISA is a sandwich immunoassay. Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples.
Specificity	The assay uses anti idiotypic (humanized) antibody for capture and / or recombinant protein expressed in mamalian cells, The standards used are research grade biosimilars referenced to the innovator product. In specfic cases where vailable the kit has also been validated against the innovator / reference product. Refer our detailed validation guide for the same.
Detection Method	Colorimetric, Absorbance measured at 450nm
Conjugate-Enzyme Reaction	Uses stable HRP Enzyme Conjugate with (single component kinetic) and TMB Chromogenic Substrate for color development.
Regulatory Status	Research Use Only. Kit is developed and validated as per ICH/EMA/FDA guidelines. Ask for our detailed validation guide at sales1@krishgen.com
Shipping Temperature	Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius
Storage Temperature	The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity.
ELISA Type	Sandwich ELISA, Double Antibody
ELISA Interpretation	Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results.
Shelf Life Available	8 -10 months at time of shipping
Alternate Names / Synonyms	Anti Abaloparatdie (TYMLOS)
Therapeutic / Biology Type	General
Sample Dilution	Dilute at 1:1000 with the Sample Diluent provided in the kit. Please refer to the IFU enclosed with the kit supplied for specific guidance.
Biological Pathway	Anti‑Abaloparatide antibodies (ADA; PTHrP agonist)
Active Molecule	Anti Abaloparatdie (TYMLOS)
Standards Available	0; 31.25; 62.5; 125; 250; 500; 1000; 2000 ng/ml
Calibration Curve	6/8 point; standards available
Disclaimer	The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit. Trade name indicated is for reference purposes only. It does not reflect any licences or patent usage. The tradename is the registered trademark of the respective owners only. References towards clinical trials, pathways, research areas are for informational purpose only.