Background: Growth differentiation factor 15 (GDF15), also known as MIC-1, is a secreted member of the transforming growth factor (TGF)-beta superfamily as a novel antihypertrophic regulator in the heart. GDF-15/GDF15 is not expressed in the normal adult heart but is induced in response to conditions that promote hypertrophy and dilated cardiomyopathy and is highly expressed in the liver. GDF-15 / GDF15 plays a role in the regulation of inflammatory and apoptotic pathways in damaged tissues and during disease processes. GDF-15/GDF15 are synthesized as precursor molecules that are processed at a dibasic cleavage site to release the C-terminal domains, which contain 7 conserved cysteines characteristic of the mature protein motif. GDF-15/GDF15 overexpression due to enlarged erythroid compartment contributes to iron overload in thalassemia syndromes by inhibiting hepcidin expression..The KRIBIOLISA Inhibitor Assay Kits available enable researchers to test the inhibitory effects of various compounds on specific enzymes at a higher output. These assays can help identify or characterize enzyme inhibitors, which is important in early drug discovery and development. They can also be useful in characterizing enzyme activity. These Inhibitor assay kits contain a known inhibitor as a control.
This Assay kit is designed to facilitate the identification and characterization of GDF15 pathway inhibitors.
Assay Features include:
– Ready to use protocol with pre-coated breakapart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision
Validated against seven points for a Gold Ring Standard Quality – the benchmark sign for Krishgen quality.
The kits are used for assessing the specific biomarker in samples analyte which may be serum, plasma, biological fluids and cell culture supernatant.
Unlike competitor assays available, we offer pre-coated ready-to-use assays to ensure high level of reproducibility and accuracy.