Description
Introduction:
IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survivability of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes. Recombinant human IL-6 is a 20.9 kD protein containing 184 amino acids. The KRIBIOLISA™ Human IL-6 ELISA (Site I/II) is a sandwich ELISA format which uses a capture antibody that recognizes and binds binding site III of human interleukin-6 which interacts with gp130 (IL-6 receptor subunit beta).
Long Name: Interleukin 6
Entrez Gene IDs: 3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names: B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
Intended Use:
The KRIBIOLISA™ Human IL-6 (Site III Binding) ELISA is specifically designed for the accurate quantitation of human IL-6 from human serum and plasma. It is ready-to-use, accurate, and sensitive.
Principle:
This assay is based on the Sandwich ELISA procedure. Samples containing human IL-6 react with already coated affinity purified capture anti-human IL-6 antibody and bind to them. Plates are washed with wash buffer to remove unbound reactants. Biotinylated Anti-human IL-6 is added leading to formation of a sandwich complex of solid phase antibody-human IL-6-biotin labeled antibody. The wells are washed to remove any unbound reactants as per the wash procedure.
Streptavidin:HRP conjugate is added which binds to Biotinylated Anti-human IL-6 complex. The wells are washed to remove any unbound reactants as per the wash procedure. The substrate 3, 3,5, 5 Tetra Methyl Benzidine is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the concentration of Human IL-6 present in the samples”