Background: Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4/IL-13 family. Interleukin-4/IL4 has many biological roles, including stimulation of the proliferation of activated B and T cells. It increases both the secretion and cell surface expression of IgE and IgG1. Interleukin-4/IL4 also regulates the expression of the low-affinity Fc receptor IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is required for the transition of B cells to the production of IgE antibodies and the maturation of T helper (Th) cells towards a Th2 phenotype. It is involved in at least several activation processes of B cells as well as other cell types. However, studies show that a double mutant (Q116D, Y119D) of murine IL4 protein (QY), and glutamine 116 and tyrosine 119, which bind to IL4 receptor alpha, completely prevent IL4-induced IL4 protein upregulation in a dose-dependent manner. lipopolysaccharide-stimulated murine splenic B cells, from the murine T-cell line CTLL-2 and from the murine pre-B cell line BA/F3. QY also prevented IL4-stimulated upregulation of CD23 expression in lipopolysaccharide-stimulated mouse splenic B cells and abolished tyrosine phosphorylation of transcription factor Stat6 and tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells..The KRIBIOLISA Inhibitor Assay Kits available enable researchers to test the inhibitory effects of various compounds on specific enzymes at a higher output. These assays can help identify or characterize enzyme inhibitors, which is important in early drug discovery and development. They can also be useful in characterizing enzyme activity. These Inhibitor assay kits contain a known inhibitor as a control.
This Assay kit is designed to facilitate the identification and characterization of IL-4 pathway inhibitors.
Assay Features include:
– Ready to use protocol with pre-coated breakapart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision
Validated against seven points for a Gold Ring Standard Quality – the benchmark sign for Krishgen quality.
The kits are used for assessing the specific biomarker in samples analyte which may be serum, plasma, biological fluids and cell culture supernatant.
Unlike competitor assays available, we offer pre-coated ready-to-use assays to ensure high level of reproducibility and accuracy.