Background: Herpesvirus entry mediator (HVEM), also known as TNFRSF14, TR2 (TNF receptor-like molecule) and ATAR (another TRAF-related receptor), is a type I transmembrane protein member of the TNF receptor superfamily. It is expressed in many immune cells, including T and B cells, NK cells, monocytes and neutrophils. Two TNF superfamily ligands, lymphotoxin alpha (TNF-beta) and LIGHT (TNFSF14), are identified as cellular ligands for HVEM and initiate positive signaling. However, recent studies have shown that HVEM also participates in a unique T-cell inhibitory signaling pathway by activating tyrosine phosphorylation of the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the suppressor of B and T lymphocytes (BTLA). HVEM provides a stimulatory signal after T-cell engagement with LUMO (TNFSF14). Conversely, it can also provide an inhibitory signal to T cells when it binds suppressor of B and T lymphocytes (BTLA), a ligand member of the immunoglobulin (Ig) superfamily. Thus, HVEM can be considered a molecular switch capable of facilitating both stimulatory and inhibitory co-signaling in T cells. Substantial evidence from both human disease and experimental mouse models has shown that dysregulation of the LIGHT-HVEM-BTLA co-signaling pathway can lead to inflammation in lung and mucosal tissues.The KRIBIOLISA Inhibitor Assay Kits available enable researchers to test the inhibitory effects of various compounds on specific enzymes at a higher output. These assays can help identify or characterize enzyme inhibitors, which is important in early drug discovery and development. They can also be useful in characterizing enzyme activity. These Inhibitor assay kits contain a known inhibitor as a control.
This Assay kit is designed to facilitate the identification and characterization of HVEM pathway inhibitors.
Assay Features include:
– Ready to use protocol with pre-coated breakapart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision
Validated against seven points for a Gold Ring Standard Quality – the benchmark sign for Krishgen quality.
The kits are used for assessing the specific biomarker in samples analyte which may be serum, plasma, biological fluids and cell culture supernatant.
Unlike competitor assays available, we offer pre-coated ready-to-use assays to ensure high level of reproducibility and accuracy.