Background: Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein and is an immunoreceptor of the CD28/CTLA-4 family that negatively regulates antigen receptor signaling by recruiting the protein tyrosine phosphatase SHP-2 when it interacts with both. Ligands, PD-L1 or PD-L2. PD1 inhibits T-cell proliferation and the production of related cytokines, including IL-1, IL-4, IL-10, and IFN-γ, by inhibiting the activation and transduction of the PI3K/AKT pathway. In addition, PD1 binding inhibits BCR-mediated signaling by dephosphorylation of a key signal transducer. PD1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance, thus contributing to the prevention of autoimmune diseases. In addition, PD1 has been shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Binding of PD-1 by its ligands PD-L1 or PD-L2 transmits a signal that inhibits T-cell proliferation, cytokine production, and cytolytic activity..The KRIBIOLISA Inhibitor Assay Kits available enable researchers to test the inhibitory effects of various compounds on specific enzymes at a higher output. These assays can help identify or characterize enzyme inhibitors, which is important in early drug discovery and development. They can also be useful in characterizing enzyme activity. These Inhibitor assay kits contain a known inhibitor as a control.
This Assay kit is designed to facilitate the identification and characterization of PD-1 pathway inhibitors.
Assay Features include:
– Ready to use protocol with pre-coated breakapart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision
Validated against seven points for a Gold Ring Standard Quality – the benchmark sign for Krishgen quality.
The kits are used for assessing the specific biomarker in samples analyte which may be serum, plasma, biological fluids and cell culture supernatant.
Unlike competitor assays available, we offer pre-coated ready-to-use assays to ensure high level of reproducibility and accuracy.