KRIBIOLISA Neutralizing Antibodies to Insulin ELISA

SKU: KBN2000

Rs 105,000.00

Enzyme Immunoassay for the estimation of Neutralizing Antibodies to Tocilizumab (ACTEMRA) in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

 

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All our kits are manufactured under ISO 13485.

Background: This ELISA detects functional neutralizing antibodies that inhibit the biological activity of the referenced therapeutic, supporting immunogenicity risk assessment and clinical monitoring.

KRIBIOLISA Neutralizing Antibodies to Tocilizumab (ACTEMRA) ELISA includes features like:
– Ready to use protocol with break-apart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Validated against seven points for a ?GOLD RING? Standard Quality ELISA – the benchmark sign for Krishgen Quality. KRIBIOLISA ELISA kit is used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant. The kit uses indirect sandwich assay with double antibodies / recombinant proteins – capture and detection to ensure a high degree of sensitivity and specificity in the estimation of the analyte. Extensive validation has been done on these kits. Please ask for our validation guide if not availableon our product page. Incase you wish us to customize the kit, connect with us at sales1@krishgen.com

Additional information

Pack Type

1 x 96 wells, break-apart wells

Species Reactivity

Homo Sapiens (Human)

Sample Type

Serum and Plasma. Note the kit has been validated using human matrices only. For any other matrix, please connect with us to optimize the kit accordingly.

Biomarker / Protein

Neutralizing Antibodies to Tocilizumab

Sample Dilution

Dilute at 1:1000 with the Sample Diluent provided in the kit. Please refer to the IFU enclosed with the kit supplied for specific guidance.

Short Name

Neutralizing Antibodies to Insulin ELISA

Assay Range

Qualitative

Standards Available

Controls – Positive, Negative

Calibration Curve

NA

Sensitivity

Qualitative

Interference

Preparations of the known factors were assayed for interference. No significant interference was observed.

Specificity

The assay uses recombinant insulin receptor (INSR/CD220) for coating and an insulin neutralizing antibody as a control.

Principle Of Assay

This ELISA is a cell-free, competitive receptor-binding neutralizing antibody format in which antibodies in the sample block insulin binding to the insulin receptor (INSR/CD220). The insulin receptor?s ligand-binding surface is in the extracellular alpha-subunit region, especially the L1 + alphaCT site, so a receptor-binding format is appropriate for NAb assessment.

Detection Method

Colorimetric, Absorbance measured at 450nm

Conjugate-Enzyme Reaction

Uses stable HRP Enzyme Conjugate with (single component kinetic) and TMB Chromogenic Substrate for color development.

Regulatory Status

Research Use Only. Kit is developed and validated as per ICH/EMA/FDA guidelines. Ask for our detailed validation guide at sales1@krishgen.com

Shipping Temperature

Shipped at Room Temperature or in gel pack

Storage Temperature

The kit and its components are to be stored at 2-8 Degree Celsius. Please refer specific components storage conditions in the IFU.

ELISA Type

Sandwich ELISA, Double Antibody

ELISA Interpretation

Qualitative Results

Shelf Life Available

8 -10 months at time of shipping

Product Catalog

Metabolic Hormones

Therapeutic / Biology Type

Metabolic / Endocrinology

Biological Pathway

Metabolic hormone signaling (PI3K?AKT)

KEGG Target Pathway

hsa04151

Reactome Pathway ID

-HSA-1257604

Active Molecule

Insulin Antibody

Research Area

Diabetes, Obesity, Metabolism

Disclaimer

The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit. Trade name indicated is for reference purposes only. It does not reflect any licences or patent usage. The tradename is the registered trademark of the respective owners only. References towards clinical trials, pathways, research areas are for informational purpose only.