Introduction: Egg allergies occur in a small size of the population. As some of the vaccines like influenza and yellow fever vaccines are made in eggs, egg proteins (primarily Ovalbumin) are present in the final product. Residual quantities of egg proteins found in the vaccines may induce severe and rarely fatal hypersensitivity reactions in children with egg allergies. The KRIBIOLISA Ovalbumin ELISA enables estimation of Ovalbumin found as impurities in vaccines and cell culture supernatants.
Intended Use: This generic kit is intended in determining the presence of Ovalbumin in biological fluids. A highly sensitive and specific Enzyme linked immunosorbent can be used for example to control the purity of vaccines.
Principle: This assay is based on the Sandwich ELISA method. Samples containing Ovalbumin are reacted with microtiter wells pre-coated with affinity purified capture Ovalbumin antibody followed by washing and then detected using detection antibody and HRP Conjugated antibody. This immunological reaction results in formation of an immuno complex of solid phase. The wells are washed to remove any unbound reactants. The substrate 3, 3& rsquo;, 5, 5& rsquo; Tetramethyl Benzidine is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the concentration of Ovalbumin present.
