Background: Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as a mediator of the immune inhibitory receptor programmed death-1 (PD1/PDCD1) and has been shown to play a role in immune regulation. reactions and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules, and this protein contains one V-like and one C-like Ig domain in the extracellular domain, and together with PD-L2, there are two PD1. middlemen which belongs to the CD28/CTLA4 family, which is expressed on activated lymphoid cells. By binding to PD1 on activated T cells and B cells, PD-L1 can suppress ongoing T cell responses, inducing apoptosis and arresting cell cycle progression. Thus, it causes an increase in immunogenic tumor growth by increasing apoptosis of antigen-specific T cells and may contribute to immune evasion of the cancer. Thus, PD-L1 is considered a promising therapeutic target in human autoimmune diseases and malignant cancers..The KRIBIOLISA Inhibitor Assay Kits available enable researchers to test the inhibitory effects of various compounds on specific enzymes at a higher output. These assays can help identify or characterize enzyme inhibitors, which is important in early drug discovery and development. They can also be useful in characterizing enzyme activity. These Inhibitor assay kits contain a known inhibitor as a control.
This Assay kit is designed to facilitate the identification and characterization of PD-L1 pathway inhibitors.
Assay Features include:
– Ready to use protocol with pre-coated breakapart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision
Validated against seven points for a Gold Ring Standard Quality – the benchmark sign for Krishgen quality.
The kits are used for assessing the specific biomarker in samples analyte which may be serum, plasma, biological fluids and cell culture supernatant.
Unlike competitor assays available, we offer pre-coated ready-to-use assays to ensure high level of reproducibility and accuracy.