Background: Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA, or TNFSF2, is the prototypical cytokine of the TNF superfamily and a multifunctional molecule involved in the regulation of many biological processes, including cell proliferation and differentiation. , apoptosis, lipid metabolism and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and interleukin-1 (IL-1). TNF-alpha is involved in the fight against the formation of tumors, so it is considered a molecule in cancer treatment.The KRIBIOLISA Inhibitor Assay Kits available enable researchers to test the inhibitory effects of various compounds on specific enzymes at a higher output. These assays can help identify or characterize enzyme inhibitors, which is important in early drug discovery and development. They can also be useful in characterizing enzyme activity. These Inhibitor assay kits contain a known inhibitor as a control.
This Assay kit is designed to facilitate the identification and characterization of TNF-alpha pathway inhibitors.
Assay Features include:
– Ready to use protocol with pre-coated breakapart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision
Validated against seven points for a Gold Ring Standard Quality – the benchmark sign for Krishgen quality.
The kits are used for assessing the specific biomarker in samples analyte which may be serum, plasma, biological fluids and cell culture supernatant.
Unlike competitor assays available, we offer pre-coated ready-to-use assays to ensure high level of reproducibility and accuracy.