Background: Osteoprotegerin, or TNFRSF11B, is a member of the TNF receptor superfamily. This protein is a decoy receptor secreted by osteoblasts that acts as a negative regulator of bone resorption. This protein binds specifically to its ligand, the osteoprotegerin ligand, both of which are important extracellular regulators of osteoclast development. Studies of the mouse counterpart also indicate that this protein and its ligand play a role in lymph node organogenesis and vascular calcification. Alternative spliced transcripts of this gene have been reported, but their full-length nature has not been determined. Osteoprotegerin/TNFRSF11B acts as a decoy receptor for RANKL and thus opposes its function in osteoclastogenesis. This protein can inhibit osteoclast activation and promote osteoclast apoptosis in vitro. Bone homeostasis appears to depend on the local RANKL/OPG ratio. Osteoprotegerin/TNFRSF11B also plays a role in preventing arterial calcification by acting as a decoy receptor for TRAIL and protecting against apoptosis. Binding of TRAIL prevents inhibition of osteoclastogenesis.The KRIBIOLISA Inhibitor Assay Kits available enable researchers to test the inhibitory effects of various compounds on specific enzymes at a higher output. These assays can help identify or characterize enzyme inhibitors, which is important in early drug discovery and development. They can also be useful in characterizing enzyme activity. These Inhibitor assay kits contain a known inhibitor as a control.
This Assay kit is designed to facilitate the identification and characterization of Osteoprotegerin pathway inhibitors.
Assay Features include:
– Ready to use protocol with pre-coated breakapart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision
Validated against seven points for a Gold Ring Standard Quality – the benchmark sign for Krishgen quality.
The kits are used for assessing the specific biomarker in samples analyte which may be serum, plasma, biological fluids and cell culture supernatant.
Unlike competitor assays available, we offer pre-coated ready-to-use assays to ensure high level of reproducibility and accuracy.