Background: VEGFR2, also called KDR or Flk-1, has been identified as a receptor for VEGF and VEGFC and an early marker of endothelial cells, with expression limited to endothelial cells in vivo. VEGFR2 has been shown to be a primary signal transducer in angiogenesis and the development of pathological conditions such as cancer and diabetic retinopathy. VEGFR2 has been shown to be mainly expressed in endothelial cells and expression increases in tumor vasculature. Thus, inhibition of VEGFR2 activity and its downstream signaling are important targets for the treatment of angiogenesis-related diseases. VEGFR2 transmits the most important angiogenesis signals through its potent tyrosine kinase activity. Unlike other typical tyrosine kinase receptors, VEGFR2 does not utilize the Ras pathway as its primary downstream signaling, but rather the phospholipase C-protein kinase C pathway to signal mitogen-activated protein (MAP) kinase activation and DNA synthesis. VEGFR2, in cooperation with many other signaling partners, is a direct and important signal transducer for pathological angiogenesis, including cancer and diabetic retinopathy; thus, VEGFR2 and its downstream signaling appear to be critical targets in suppressing these diseases. VEGF- and VEGFR2-mediated survival signaling is critical for endothelial cell survival, maintenance of vascular and alveolar structures, and lung tissue regeneration. Decreased expression of VEGF and VEGFR2 in emphysematous lungs was associated with increased endothelial cell death and vascular regression..The KRIBIOLISA Inhibitor Assay Kits available enable researchers to test the inhibitory effects of various compounds on specific enzymes at a higher output. These assays can help identify or characterize enzyme inhibitors, which is important in early drug discovery and development. They can also be useful in characterizing enzyme activity. These Inhibitor assay kits contain a known inhibitor as a control.
This Assay kit is designed to facilitate the identification and characterization of VEGFR2 pathway inhibitors.
Assay Features include:
– Ready to use protocol with pre-coated breakapart wells for ease of use
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision
Validated against seven points for a Gold Ring Standard Quality – the benchmark sign for Krishgen quality.
The kits are used for assessing the specific biomarker in samples analyte which may be serum, plasma, biological fluids and cell culture supernatant.
Unlike competitor assays available, we offer pre-coated ready-to-use assays to ensure high level of reproducibility and accuracy.