KRISHGEN rcAAV-2/N Quantitation RT-PCR Kit

SKU: KBPL9011

Rs 218,300.00

Assay for the estimation of rcAAV-2/N in cell culture supernatant and other biological preparations


Availability: 3 – 4 Weeks | Pack Size: 1 x 96 wells, break-apart

Free worldwide shipping on all orders over $3000

 

 

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All our kits are manufactured under ISO 13485.

The KRIBIOLISA Bioprocess Assay Kits available enable researchers to estimate and measure the amount of process analyte left during the production process. These assays can help identify or characterize the residual analyte which is important in early process, downstream purification and final product release.

This Assay kit is designed to facilitate the identification and characterization of analytes used in production and purification.

Assay Features include:
– Ready to use protocol with ease of use components
– Standardisation and High Reproducibility
– Lot to Lot Consistency
– Accuracy and Precision

Validated against seven points for a Gold Ring Standard Quality – the benchmark sign for Krishgen quality.
The kits are used for assessing the specific biomarker in samples analyte which may be cell culture supernatant and other biological preparations.
Unlike competitor assays available, we offer pre-coated ready-to-use assays to ensure high level of reproducibility and accuracy and also offer custom assay development which is process specific to comply with FDA needs from our ISO13485 certified factory and labs.

Additional information

Pack Type

200 reactions

Species Reactivity

n/a

Sample Type

Cell Culture Supernatant and other biological samples

Assay Range

2 million copies / ul

Sensitivity

0.4 copy / ul

Interference

Preparations of the known factors were assayed for interference. No significant interference was observed.

Specificity

The primers in the kit are specific for the analyte to be measured using the standards available in the kit for quantification of the results.

Principle Of Assay

Real-time PCR monitors the accumulation of PCR product (amplicon) cycle-by-cycle using a fluorescent signal that increases proportionally with the amount of amplified DNA.

Detection Method

Fluorescent Signal

Real Time PCR Reaction

separating dye from quencher., Sequence-specific probes with reporter and quencher; probe is cleaved by Taq polymerase during amplification

Quality Certification

Validated as per KRISHGEN's Gold Ring 7-Point Program

KRISHGEN Mandate

High Performance Assays at Affordable Prices

Regulatory Status

Research Use Only.

Shipping Temperature

-20 Degrees Celsius, dry ice

Storage Temperature

if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, The kit and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, well sealed and store at the indicated temperature.

Assay Type

Biorad, Qiagen., Real Time PCR assay. Instrument compatability to Thermo, Roche

Assay Interpretation

Ct Value), on request, Quantitative Results Intepretation (Amplification Plot, Software (MS Excel) available, to support interpretation of results.

Shelf Life Available

8 -10 months at time of shipping

Alternate Names / Synonyms

rcAAV-2/N Quantitation

Research Area

Cell Gene Therapy

Disclaimer

The data indicated herein with specifications are changed from time to time at time of production. Please refer the most current IFU (instructions for use) accompanying the kit.