The Krishzyme KriGly N-Glycan Kit is a plug-and-play kit th integrates workflows in 3-steps. The 3-step workflow includes i) Rapid Deglycosylatio, ii) 5-minute Labeling, and iii) Cleanup.
Why Biopharma Core Labs Should Switch to Krishgen: Go from Sample to Data in under an hour: – Complete deglycosylation and labeling happen in minutes, not hours.
– Streamlined Sample Prep: Skips the evaporation and drying steps required by legacy methods.
– Massive Mass Spec Boost: Our proprietary amine-tail design forces labeled glycans to ionize instantly, revealing hidden, low-abundance peaks.
– Seamless Instrument Dropping: Matches standard excitation (265 nm) and emission (425 nm) channels perfectly to leading Competitors.
The Krishzyme KriGly N-Glyan kit features the KriGly Fluorophore Dye with a strategically modified chemical structure. It provides full freedom to operate without infringing on Waters Corporation’s active composition patents.
Mass Spectrometry Adjustment Notice: A one-time adjustment in the data analysis software is suggested. Because the KriGly Fluorophore Dye has a tail module which is structurally modified to keep it legally distinct, it carries a unique molecular weight. Users have to simply update their target m/z extraction lists to perfectly match the new label.
Formula for Expected Mass (m/z) Calculation:
Target Monoistopic Mass = Native Glcan Mass + KriGly Label Mass Shift
Example Target Mass Setup Table for Common IgG Glycans:
Glycan ID Structure Native Monoisotopic Mass (Da) Waters* RFMS Target Mass (+309.14 Da) Krishgen Target Mass (Refer to CoA)
G0F FA2 1445.53 1754.67 1445.53 + Krishgen Shift
G1F FA2G1 1607.58 1916.72 1607.58 + Krishgen Shift
G2F FA2G2 1769.64 2078.78 1769.64 + Krishgen Shift
Key Features:
Single Column / 96-well plate Workflows
Designed for 1 column / 96-well format processing, enabling parallel sample preparation and improved workflow efficiency in batch analysis.
Optimized 3-step Process
Integrates enzymatic digestion, labeling, and SPE-based enrichment/purification into a simplified workflow suitable for high-throughput applications.
Rapid Enzymatic Digestion
PNGase F digestion can be completed in approximately 5 minutes at 50°C, under recommended conditions.
Quick and High Sensitivity Labeling Reaction
Labeling reaction can be completed in approximately 5 minutes at room temperature. The Label Dye optimized for high sensitive applications equivalent to market competitors.
Efficient SPE-based Purification
Solid-phase extraction provides a typical recovery rate of >90%, with a processing time of ≤10 minutes per sample. When combined with a 96-well plate and vacuum manifold system, up to 12 samples can be processed in approximately 1.5 hours under optimized conditions.
Direct Compatibility with LC/LC-MS analysis
SPE-prepared samples can be directly used for LC or LC-MS analysis without additional purification steps.
Applications:
High-throughput N-Glycan Profiling
Designed for parallel preparation and analysis of N-linked glycans from glycoproteins in 96-well format workflows.
Batch Sample Preparation for Glycan Analysis
Suitable for laboratories requiring efficient processing of multiple samples for LC or LC-MS-based glycomics studies.
LC/LC-MS-based Glycan Characterization
Applicable in liquid chromatography and mass spectrometry-based N-glycan analysis for research applications.
FAQs:
Do I adjust the protein concentration in the sample, while keeping the sample volume the same?
Yes, keep the sample addition volume of 20ul, as this may affect the performance of the reaction system. Should you desire, adjust the protein concentration to achieve the desired input amount.
How should the labeling reagent be handled after reconstitution?
The labeling reagent is recommended for immediate use after dissolution. If aliquots are required, do it immediately after reconstitution. The aliquots maybe stored at -80 °C for up to 5-7 days.
Is precipitation normal after adding acetonitrile?
Yes. The presence of slight precipitation after adding acetonitrile is normal and is caused by phase incompatibility between aqueous and organic solvents. This does not typically affect the reaction performance under recommended conditions.
Can the recommended sample volume be adjusted?
The recommended sample volume is 200ul. This can be adjusted according to specific sample conditions and instrument requirements. Optimization may be required depending on experimental setup.
