The PrimePlex single-plex enable quantitative measurement in serum, plasma, tissue homogenates, cell culture supernatants, and other biological fluids. Each kit includes analyte-specific magnetic bead panels, standards, detection reagents, and buffers for a streamlined 3.5 – 5 hour workflow on standard flow cytometers (liquid-based and plate based). Assay ranges are analyte-specific, derived from validated single-analyte, ensuring reliable linearity, <10?15% intra/inter-assay CV%, and minimal cross-reactivity. A key benefit of the PrimePlex single-plex Assay kits is the flexibility to mix-and-match these into custom multiplex panels (up to 8-plex), allowing tailored configurations for specific research needs like cytokine profiling or biomarker panels without compromising performance. This modular approach leverages proven single-analyte validation for consistent results across custom combinations. Pick and choose the analytes as per your requirement to make the kit (upto 8-plex)and order it from us.
PrimePlex Single Plex Kit for Vaccinia Related Kinase 1 (VRK1)
SKU: KPZHU600
Rs 91,200.00
PrimePlex Single Plex Assay Kit is a customizable multiplex solution from Krishgen that allows you to combine multiple singleplex analytes into one tailored assay panel. Based on a magnetic bead?based sandwich immunoassay format, each kit is developed to match your specific research requirements. Due to this being a customised assay kit, please allow us 4 – 6 weeks to develop and deliver the kit to you.
Designed for in vitro quantitative analysis, the assay enables accurate detection of target analytes in species-specific samples, including serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids.
Additional information
| Pack Size | 96 tests |
|---|---|
| Species Reactivity | Homo Sapiens (Human) |
| No of Multiplex | 1-Plex |
| Target Analyte(s) | Vaccinia Related Kinase 1 (VRK1) |
| Sample Type | Serum, Plasma, Tissue Homogenates, Cell Culture Supernatant and other biological samples. |
| Respective Analyte Component Cat No | Single-plex Kit, the Cat No is the kit Cat No only. |
| Assay Range | 0.01 – 10 ng/ml |
| Sensitivity | 0.003 ng/ml |
| Interference | Preparations of the known factors were assayed for interference. No significant interference was observed. |
| Specificity | This assay has high sensitivity and excellent specificity for detection of the target analytes by FLIA (Flow Luminescence Immunoassay). No significant cross-reactivity or interference between the target analytes and analogues was observed. |
| Principle Of Assay | Single-Plex Double-antibody Sandwich Immunoassay |
| Detection Method | The assay is read on a compatible flow cytometer, where dual-laser excitation identifies each bead population by internal fluorescence and quantifies analyte concentration via a reporter fluorescence intensity (reported as MFI). |
| Components Included in the Kit | 96-well microplate – 1no; Pre-Mixed Standard – 2 vials; Pre-Mixed Magnetic Beads – 1x1ml; Setup Beads – 0.4ml; Standard Diluent – 1x20ml; Analysis Buffer – 1x20ml; Pre-Mixed Detection Reagent A – 1x120ul; Detection Reagent B – 1x120ul; Assay Diluent A – 1x12ml; Assay Diluent B – 1x12ml; (30X) Wash Buffer – 1x20ml; Sheath Fluid – 1x10ml |
| Flow Cytometer Instrument Applicability | The kit may be used on flow cytometry systems like NovoCyte D2060R, Navios, FACSMelody, Gallios, DxFLEX, CytoFLEX S, NovoCyte D2060R, Navios, FACSMelody, Gallios, DxFLEX, CytoFLEX S, etc. or plate based Luminex instruments such as Luminex MAGPIX, Luminex 100, Luminex 200, FLEXMAP 3D, BioFlex, etc |
| Assay Interpretation | The Median MFI (Median Fluorescence Intensity) value of each standard, control and samples is calculated by subtracting the zero standard MFI value. Create standard curves with target analyte concentration on the Y-axis and corresponding MFI on the X-axis, respectively. Draw a best fit curve through the points and it can be determined by regression analysis using a 4PL (2nd order) or Cubic Spline Graph software. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. |
| Regulatory Status | For Research Use Only (RUO). |
| Shipping Temperature | Shipped in Dry Ice to maintain temperature at -20 Degrees Celsius |
| Storage Temperature | Store the kit at -20 Degrees Celsius |
| UniProt ID | Q99986 |
| Research Area | Enzyme & Kinase; |
| Disclaimer | The data indicated herein with specifications are changed from time to time at time of production. Please refer the most current IFU (instructions for use) accompanying the kit. |